Journal: bioRxiv

Article Title: Identification of a proteolysis regulator for an essential enzyme in Mycobacterium tuberculosis

doi: 10.1101/2024.03.29.587195

Figure Lengend Snippet: Cryo-EM data processing workflow. (A) Cryo-EM data processing pipeline. (B) Representative cryo-EM micrograph. Particles of interest are circled in white. Scale bar (100 nm) is indicated on the bottom left of the micrograph. (C-D) Representative 2D class averages showing different views of the particles were generated in cryoSPARC using the final set of particles extracted with box size of 256 pixels and no binning for CoaX Mtb hexamer complex ( c ) and CoaX Mtb tetramer complex ( d ).

Article Snippet: Selected cryo-EM grids were imaged at NYU Cryo-EM Laboratory on a Krios-3Gi operated at 300 kV with a K3 camera (Gatan) and an energy filter slit width of 20 eV.

Techniques: Cryo-EM Sample Prep, Generated

Journal: bioRxiv

Article Title: Identification of a proteolysis regulator for an essential enzyme in Mycobacterium tuberculosis

doi: 10.1101/2024.03.29.587195

Figure Lengend Snippet: Cryo-EM validation and representative densities. (A) CoaX Mtb hexamer map (contour level: 0.25) colored by local resolution that was estimated in cryoSPARC . (left) Whole structure view. (right) Cross-sectional view. (B) Gold-standard FSC curve calculated in cryoSPARC for raw maps of CoaX Mtb hexamer. The dotted line represents the 0.143 FSC cutoff. (C) Directional 3DFSC calculated for the CoaX Mtb hexamer map. (D) Examples of cryo-EM densities (transparent gray surface) with corresponding areas of the model (sticks) are shown from each subunit for the CoaX Mtb hexamer map. Protein densities rendered using ChimeraX 3 ‘volume zone’ with 2.0 Å distance cutoff around the indicated protein residues with the following contour levels: ChainA (0.6); ChainB (0.3), ChainC (0.4), ChainD (0.6), ChainE (0.3), ChainF (0.4). (E) CoaX Mtb tetramer map (contour level: 0.3) colored by local resolution that was estimated in cryoSPARC. (left) Whole structure view. (right) Cross-sectional view. (F) Gold-standard FSC curve calculated in cryoSPARC for raw maps of CoaX Mtb tetramer. The dotted line represents the 0.143 FSC cutoff. (G) Directional 3DFSC calculated for the CoaX Mtb tetramer map. (H) Examples of cryo-EM densities (transparent gray surface) with corresponding areas of the model (sticks) are shown from each subunit for the CoaX Mtb tetramer map. Protein densities rendered using ChimeraX ‘volume zone’ with 2.0 Å distance cutoff around the indicated protein residues with the following contour levels: ChainA (0.25); ChainB (0.3), ChainC (0.3), ChainD (0.6).

Article Snippet: Selected cryo-EM grids were imaged at NYU Cryo-EM Laboratory on a Krios-3Gi operated at 300 kV with a K3 camera (Gatan) and an energy filter slit width of 20 eV.

Techniques: Cryo-EM Sample Prep

Journal: ACS Omega

Article Title: Cu/CuO-Doped ZnO Nanocomposites via Solution Combustion Synthesis for Catalytic 4-Nitrophenol Reduction

doi: 10.1021/acsomega.3c00141

Figure Lengend Snippet: ZnO NPs’ transmission electron microscopy (TEM) morphological analysis: (a) In the TEM image, the presence of some pores is observed. (b) In the SAED ring image, the spots on the ring indicate the crystallinity; the inset in panel b is the XRD pattern. (c) In the high-resolution TEM image, the d-spacing analysis shows that all the crystallites are for ZnO NPs.

Article Snippet: Scanning electron microscopy (SEM, JCM-6000Plus), transmission electron microscopy (TEM), selected-area electron diffraction (SAED), and high-resolution TEM (HRTEM) (JEOL TEM 2100 HRTEM) showed the porosity, crystallinity, and morphology of the materials.

Techniques: Transmission Assay, Electron Microscopy

Journal: ACS Omega

Article Title: Cu/CuO-Doped ZnO Nanocomposites via Solution Combustion Synthesis for Catalytic 4-Nitrophenol Reduction

doi: 10.1021/acsomega.3c00141

Figure Lengend Snippet: The 10% doped NCs underwent transmission electron microscopy (TEM) morphological analysis: (a) In the TEM image, the presence of pores is observed due to the evolution of gases. (b) In the SAED ring image, the spots on the ring indicate the crystallinity; the inset in panel b is the XRD pattern. (c) In the high-resolution TEM image, the d-spacing analysis shows the presence of both ZnO and CuO NP crystallites.

Article Snippet: Scanning electron microscopy (SEM, JCM-6000Plus), transmission electron microscopy (TEM), selected-area electron diffraction (SAED), and high-resolution TEM (HRTEM) (JEOL TEM 2100 HRTEM) showed the porosity, crystallinity, and morphology of the materials.

Techniques: Transmission Assay, Electron Microscopy

Journal: Applied Physics A

Article Title: Double hysteresis (P-E) loops in the transparent composite containing BaTiO3 at room temperature

doi: 10.1007/s00339-024-07495-7

Figure Lengend Snippet: Fig. 3 a–d The transmission electron microscope (TEM) images along with the selected area electron diffraction (SAED) patterns of the present transpar- ent composite sample at first and second regions, respectively

Article Snippet: The transmission electron microscopy (TEM), selected area electron diffraction (SAED), and high-resolution transmission electron microscopy (HR-TEM) studies of the present transparent composite sample were carried out using a JEOL model JEM2100 PLUS with LaB6 source operating at an accelerating voltage of 200 kV.

Techniques: Transmission Assay, Microscopy

Journal: Applied Physics A

Article Title: Double hysteresis (P-E) loops in the transparent composite containing BaTiO3 at room temperature

doi: 10.1007/s00339-024-07495-7

Figure Lengend Snippet: Fig. 5 a–d The shape of the particle of BaTiO3 nanopar- ticles/clusters in the present transparent composite sample, the distribution of particle size histogram, and the HR-TEM image taken from the region in Fig. 3c, and the SAED pattern taken from the region in Fig. 5c, respectively

Article Snippet: The transmission electron microscopy (TEM), selected area electron diffraction (SAED), and high-resolution transmission electron microscopy (HR-TEM) studies of the present transparent composite sample were carried out using a JEOL model JEM2100 PLUS with LaB6 source operating at an accelerating voltage of 200 kV.

Techniques:

Journal: Nanoscale Advances

Article Title: In situ formation and superior lithium storage properties of tentacle-like ZnO@NC@CNTs composites †

doi: 10.1039/c8na00228b

Figure Lengend Snippet: SEM images of (a, b) ZnO@NC and (c, d) ZnO@NC@CNTs. (e–g) TEM images of theZnO@NC@CNTs network at different magnifications. (h, i) HR-TEM images of the ZnO@NC@CNTs at the selected area in (g). (j) Elemental mapping images of an individual ZnO@NC@CNTs polyhedron. Red, green, purple, and cyan are associated with carbon, nitrogen, cobalt, and zinc elements, respectively. (k) TEM picture of a selected CNT in the ZnO@NC@CNTs. The inset of (c) is an enlarged picture of an individual ZnO@NC@CNTs polyhedron. The inset of (f) is the SAED image. The red arrow in (i) and blue arrow in (k) point to amorphous carbon layers and ZnO inside the CNTs, respectively.

Article Snippet: X-ray diffraction (XRD, Bruker, D8-Advance), field-emission scanning electron microscopy (FESEM, JEOL, SU8010), transmission electron microscopy (TEM), high-resolution TEM (HR-TEM), selected area electron diffraction (SAED, performed on Tecnai G2 F20 S-TWIN), thermogravimetric analysis (TGA, TA-Q50), N 2 adsorption/desorption isotherms (Quantachrome, Autosorb-IQ2-VP), X-ray photoelectron spectroscopy (XPS, Thermo ESCALAB 250XI), and Raman spectroscopy (Raman, LabRAMHR800, 532 nm) were employed for morphological and structural investigations.

Techniques: